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STA20
Sigma
Starch (GO/P) Assay Kit
sufficient for 20 assays
Description
| Application | This kit is for the quantitative, enzymatic determination of starch in food and other materials. The hydrolysis of starch to glucose is catalyzed by α-amylase and amyloglucosidase. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration. |
Properties
| usage | sufficient for 20 assays |
| storage temp. | 2-8°C |
Safety
| Hazard Codes | T |
| Risk Statements | 45-22-36/37/38-42 |
| Safety Statements | 53-26-36/37/39-45 |
| RIDADR | UN 3316 9/PG 3 |
Components
| Standard component | α-Amylase, Heat Stable 0.5 ml |
| Starch Assay Reagent 1 vial | |
| Glucose Oxidase/Peroxidase Reagent 1 capsule | |
| o-Dianisidine Reagent 5 mg | |
| Glucose Standard Solution 0.5 ml | |
| Wheat Starch 1 g | |
| Corn Starch 1 g |
References
| reference | Am. Assoc. Cereal Chem. Approved Methods 9th ed.,, 76-12, (1995) |
| Karkalas, J. J. Sci. Food Agric. 36, 1019, (1985) |
